Seed Longevity Research

Astragalus bibullatus in Fruit. Attempts to establish a new population of A. bibullatus began in 2001.

How Long Do Rare Seeds Live in Frozen Storage?

植物绝种率高,促使植物园将种子库作为一种成本效益高的保护策略. Thus, the CPC National Collection 主要以种子的形式保存在植物园中零下18摄氏度的“传统”冷藏库中. CPC的种子银行旨在让种子存活几十年或几个世纪,以防止野生种群的减少. Because most research has been conducted on crop species, no one actually knows how long many rare plant seeds can survive in seed banks

To ensure better stewardship of rare plants, CPC’s national office, its network of botanic gardens, 和国家遗传资源保护实验室(NLGRP)最近获得了博物馆和图书馆服务研究所国家领导基金(mg -245983- ms -20),以以一种新的方式测试野生稀有植物物种种子的老化和长寿. 最近对作物物种的研究表明,生物化学指标可以可靠地测量种子老化和预测种子寿命. RNA integrity number (RIN) has shown particular promise as a metric of seed aging. Applying this new technology to wild species will be especially useful for curators, given the enormous variation wild seeds display in germination requirements and storage behavior.

Abies fraseri
Abronia umbellata var. brevifolia
Agalinis skinneriana
Aletes humilis
Amaranthus pumilus
Amelanchier nantucketensis
Amorpha herbacea var. crenulata
Amsonia tharpii
Anemone patens var. multifida
Arctostaphylos catalinae
Aster furcatus (Eurybia furcata)
Astragalus albens
Astragalus bibullatus
Astragalus cremnophylax var. hevronii
Astragalus hypoxylus
Astragalus linifolius
Astragalus magdalenae var. peirsonii
Astragalus tegetarioides
Berberis nevinii
Besseya bullii
Bidens torta
Boechera parishii
Bonamia ovalifolia
Calochortus umpquaensis
Campanula scabrella
Carex comosa
Carex oronensis
Castela emoryi
Castilleja kaibabensis
Castilleja levisecta
Ceanothus cyaneus
Ceanothus roderickii
Charpentiera elliptica
Chrysopsis floridana
Cimicifuga elata
Cirsium pitcheri
Clarkia biloba ssp. australis
Clarkia lewisii
Clematis socialis
Clermontia kakeana
Cordylanthus maritimus ssp. palustris
Cyanea angustifolia
Dalea foliosa
Deinandra increscens ssp. villosa
Deinandra mohavensis
Dicerandra immaculata
Dodonaea viscosa
Dubautia menziesii
Echinacea tennesseensis
Echinocactus horizonthalonius var. nicholii
Eragrostis variabilis
Erigeron parishii
Eriogonum crosbyae
Eutrema penlandii
Festuca ligulata
Geum geniculatum
Gilia leptantha ssp. leptantha
Hedeoma diffusum
Hesperocyparis abramsiana
Hesperocyparis forbesii
Hibiscus dasycalyx
Horkelia hendersonii
Hymenoxys texana
Kalmiopsis fragrans
Lasthenia burkei
Leiophyllum buxifolium
Liatris novae-angliae
Lilium parryi
Linum carteri var. carteri
Lomatium bradshawii
Lupinus westianus var. aridorum
Lycium sandwicense
Malacothamnus clementinus
Metrosideros polymorpha var. polymorpha
Nolina brittoniana
Orcuttia californica
Osteomeles anthyllidifolia
Oxypolis canbyi
Oxytropis campestris var. chartacea
Packera franciscana
Paysonia stonensis
Penstemon clutei
Penstemon peckii
Penstemon shastensis
Phacelia formosula
Physaria globosa
Physaria obcordata
Pityopsis ruthii
Plagiobothrys hirtus
Poa mannii
Pogogyne nudiuscula
Polemonium eddyense
Polemonium occidentale ssp. lacustre
Polyscias racemosa
Ptilimnium nodosum
Purshia subintegra
Remirea maritima
Remya kauaiensis
Rhus kearneyi ssp. kearneyi
Sarracenia oreophila
Schoenoplectus tabernaemontani
Senecio ertterae
Sesbania tomentosa
Sidalcea nelsoniana
Solidago plumosa
Tephrosia angustissima var. corallicola
Thermopsis macrophylla
Tumamoca macdougalii
Vaccinium boreale
Vaccinium crassifolium ssp. sempervirens
Warea amplexifolia
Ziziphus celata

Goal of Study & Accession Definitions

本研究的主要目标是比较冷冻保存15年以上的种子的发芽活性和RNA完整性,以及从同一植物群体中重新收集或再生的种子. 我们正在测试的假设是,RNA完整性或(RIN)可以线性代理存储中的种子健康,可用于为围绕稀有植物的管理和管理决策提供信息.   Please see grant project narrative for full details.

For simplicity, hereafter, 目前长期保存的种子被称为“原始种子”,参与者重新收集或再生的种子被称为“新鲜种子”。.

Partner Instructions

Species Target List & Seed Quantities

  • Make one “fresh” seed collection from each target species listed in your contract. Fresh collections should either be made from the same population as the “original accessions”; from ex-situ plants of shared wild provenance; or from plants grown or bulked from seeds of a seed collection held in long term storage for 15 years or more – known as the “original accession.“测试所需种子的具体数量可能会根据种子的大小和不同实验组的包含物而有所不同. 对研究中忆起的批准物种或物种名录的更改必须事先通过电子邮件与CPC网上信誉赌博下注人进行批准, Dr. Katie Heineman (kheineman@www.kevinangeloni.com)

Recollecting & Processing of “Fresh Accession”

  • Participants will obtain permits necessary to collect the “fresh accession”.
  • While acquiring seed for testing is a first priority, 收藏者应做一个善意的尝试,做一个完整的人口水平的保护收藏根据 CPC Best Practice guidelines, meaning, in most cases, that seed not set aside for testing should be collected & stored along maternal lines.
  • Clean, process, 并在收集后90天内将种子送到NLGRP,以确保研究中使用的“新鲜种子”具有类似的成熟度.
  • 将指定数量的用于测试的种子放在一个单独的大包装中,并与可用的母系结合在一起. 如果需要更多时间处理,参赛者可以同时或稍后在收集季节将额外的种子发送给NLGRP进行备份存储.
  • All seeds collection should be processed & sent to NLGRP no later than  August 31, 2023 (and no more than 90 days after your most recent seed collection).

Material Transfer of Fresh & Original Accessions to National Laboratory for Genetic Resources Preservation

  • Process and send fresh seeds to NLGRP within 90 days of collection to the address below. We encourage that the “original accession” be sent in advance of the fresh collection if possible, but both should arrive at least concurrently:
    • Chris Walters and Lisa Hill
      NLGRP
      1111 South Mason St.
      Fort Collins, CO 80523-0001
  • Email CPC staff (kheineman@www.kevinangeloni.com) and Lisa Hill at NLGRP (lisa.hill@ars.usda.gov),以确保NLGRP能够收到您的包裹,并验证有足够数量的种子用于测试.
  • 使用网上信誉赌博下注网站的网上信誉赌博下注成员资源部分的在线NLGRP提交表格,提交与每次加入相关的数据(http://www.kevinangeloni.com/dashboard/resources).
  • 将测试用的种子以单一的大包装发送,并与母系整合在一起. 如果需要更多的处理时间,参赛者可以同时或稍后在收集季节将额外的种子发送给NLGRP进行备份存储.
  • 首次从CPC参与机构为本研究发送的新鲜和冷冻将根据CPC-NLGRP材料转移研究协议进入NLGRP集合. Details regarding this agreement can be found here.
    • 发放由NLGRP(又名“研究仓库”又名MTRA)根据材料转让研究协议保管的种子,以供测试, contact Lisa Hill: lisa.hill@ars.usda.gov
    • 释放目前在NLGRP(又名“黑箱存储”又名MTA)根据材料转让协议持有的种子进行测试, contact Stephanie Greene: Stephanie.Greene@ars.usda.gov
    • Email CPC staff (kheineman@www.kevinangeloni.com) and Lisa Hill at NLGRP (lisa.hill@ars.usda.gov),以确保NLGRP能够收到您的包裹,并验证有足够数量的种子用于测试.
  • Seeds should arrive at NLGRP no later than August 31, 2023 (and no more than 90 days after your most recent seed collection).

Reporting & Invoicing

  • Prior to submitting your invoice for collections, collectors must ensure that:
    • 柯林斯堡NLGRP的工作人员已经收到了“原始”和“新鲜”的测试包
    • Data for the fresh recollection has been entered through the NLGRP submission form on the IMLS
    • 你已将每一契约物种的一张照片及一份保育更新资料上载至CPC网站 www.kevinangeloni.com/dashboard 
      • “保护更新”将包括一段叙述描述的回忆或种子膨胀过程或教训,通过过去的种子管理,该物种.  Any photo of the species biology (flowers, fruits, habitat, field work) would be acceptable.
  • Submit invoice via provided Invoice Template IMLS to info@www.kevinangeloni.com no later than September 30, 2023.
Where will the seeds be tested for RNA analysis?
      我知道这有点令人困惑,因为网上信誉赌博下注国家办公室批准了这些合同,但所有的实验室工作实际上都将在柯林斯堡的遗传资源保护国家实验室进行, Colorado in the lab of Dr. Chris Walters.
How should I process seed intended for testing prior to sending to NLGRP?
      Please process seeds intended for testing as you would for long term storage. Please clean seeds and dry them to ~30% RH at room temperature. 在收集后90天内,将用于测试的种子装入散装包装袋,并隔夜将种子运往NLGRP. You may take additional time to process the full population level seed collection.
How do I pay for shipping to NLGRP?
      There is no grant line or purchase code for shipping. 运费是2800美元/托收补偿的一部分,你将收到发票.
Do I need to track time and expenses? 
      Nope! 你只需要提供证明,证明分包的成果,概述在发票模板(附件)